FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8082586
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC575560.7120988257965953No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA465670.57613986414744No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG426390.5275415566255651No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG347580.4300356346347568No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA327600.4053158234258194No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG313630.388031751224175No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC304270.3764512991262945No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT296110.3663555203742961No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT263870.3264672964815964No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC227300.2812218762658387No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC216100.26736492503760556No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA182280.2255218812394944No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC174260.21559931437784888No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG171710.2124443835178494No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG167700.2074831000870266No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG147190.18210755815032464No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT140800.17420167258350236No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC126290.15624949737621102No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA109160.13505578536374374No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA94960.11748715077080528No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT87940.10880181169739486No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC85240.1054612966691601No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT85030.10520147883363072No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT84520.10457049266163082No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT347000.069.248478
AGTAGGT348950.069.242617
GTTGTAT358100.069.0961112
TAGTAGG349050.069.0427256
TAGGTTG348200.068.980099
GTAGTAG634950.068.959015
TTGGCGA169550.068.9425317
GAGGTAG703450.068.9056852
TGTATAG456600.068.807214
CGTAAAT187100.068.760699
TGAGGTA681950.068.757951
GGTAGTA640450.068.754794
AGCTTAT323850.068.741662
AGATGCT100300.068.73916
TATAGTT409100.068.7205816
GCTTATC323800.068.709043
CAGCACG186250.068.6986854
AGGTTGT410700.068.6658910
GTTGACA211900.068.6430518
ATTGGCG182950.068.6370416
Produced by FastQC (version 0.11.5)