FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10742247
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC819680.7630433372086863No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA528200.49170345831742646No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG518920.4830646698032544No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT342220.31857394453879156No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG339500.3160418858363618No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG335020.3118714362088304No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA307150.28592714354827253No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC256980.23922369314352945No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC255050.23742704854952598No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC248080.23093864812454976No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT240220.22362174319767553No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC204870.19071428910543575No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG201250.18734441686176084No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC182840.17020647542362413No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG174280.16223793774244813No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG167390.15582400963224918No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA157370.14649635220638663No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA152740.1421862669886477No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT130120.12112921998535316No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG108470.10097514979873393No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA107490.10006286394271142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT422900.069.405468
AGTAGGT425450.069.236247
TAGGTTG424200.069.184849
GTTGTAT435300.068.9877312
TAGTAGG426150.068.951666
CAGCACG221900.068.8738864
GAGGTAG848300.068.871512
TGAGGTA824800.068.8687741
TGTATAG548200.068.79954514
GGTAGTA773850.068.763564
AGGTTGT496600.068.7459710
AGCTTAT408050.068.7157442
GCTTATC407350.068.687783
GTAGTAG769150.068.665685
GTTGACA264100.068.6439418
AGATGCT116150.068.488086
ATTGGCG217800.068.4186516
TATAGTT493050.068.41048416
GGGCTAT74500.068.2549315
AAGATGC116650.068.2242055
Produced by FastQC (version 0.11.5)