FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9087181
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG662880.729467147182388No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA629970.6932512954237403No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC604230.6649256793718537No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG407610.44855494789858374No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG404450.44507752184093174No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT389220.42831764878459005No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA319220.3512860588998942No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC247450.27230667024239974No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT230490.253643016464622No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC208170.22908094380424468No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC194430.21396074316116295No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC160230.1763253092460687No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG160210.17630330022038737No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC154500.17001972338836435No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA141330.15552677997720085No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA140210.1542942745390457No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT134660.1481867699124734No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT108840.11977311775786131No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA103840.11427086133752591No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA103720.11413880718343786No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG96160.10581939547589071No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG94860.10438880880660349No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG93860.10328835752253641No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG92410.10169270316063915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT481900.069.485368
AGTAGGT485350.069.316297
GTTGTAT496850.069.2329912
TAGGTTG483750.069.183459
GAGGTAG948100.069.1503142
TAGTAGG485400.069.116016
CAGCACG262100.069.114354
TATAGTT560700.069.08395416
GGTAGTA862350.069.066264
TGAGGTA922400.069.056211
GTAGTAG858000.069.041165
TTGGCGA237200.069.02183517
TGTATAG631850.068.9971114
AGATGCT116300.068.94246
AGGTTGT568150.068.8541610
GTTGACA261200.068.8050418
TAGTTTG226900.068.75989
CGTAAAT264650.068.750689
AGCTTAT417100.068.745422
GCTGGCA116300.068.72986610
Produced by FastQC (version 0.11.5)