FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9689528
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG701050.7235130545058542No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA686360.708352357307807No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC603870.623219211503388No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG439190.45326253249900306No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT420430.43390142430054385No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG351860.36313430334274277No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA333290.34396928312710384No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC272380.281107604003002No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT238930.2465857986064956No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC221190.2282773732631765No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC216320.22325132865088992No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG171340.17683007882324092No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA168590.173991963282422No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC162360.16756234152994862No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC150680.15550809079657957No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT141950.14649836400699806No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA138030.14245275930881257No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT113600.1172399728861922No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA105950.10934485147264139No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG104860.10821992567646227No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG99340.10252305375452757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT500600.069.4622047
GTAGGTT498300.069.4597558
GTTGTAT518500.069.3729212
CAGCACG297450.069.2177054
TAGGTTG499700.069.2164849
TAGTAGG503000.069.0893866
TATAGTT597900.069.0818316
TGTATAG670150.069.069914
GAGGTAG1008050.069.0676352
GTTGACA369750.068.9759318
TGAGGTA979900.068.972771
AGGTTGT588200.068.9407610
GTAGTAG912050.068.931345
GGTAGTA918600.068.93134
AGCTTAT579900.068.878122
GCTTATC579400.068.859033
TTGGCGA269300.068.8394117
ATTGGCG293200.068.7535816
CGTAAAT300100.068.687769
GTATAGT672550.068.6777215
Produced by FastQC (version 0.11.5)