FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH215.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH215.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9607487
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1293431.3462729639915203No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA678710.7064386347855584No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG673870.7014008970295771No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG428620.44613123077866257No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT425940.4433417396245241No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC410890.42767687325520193No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA395270.4114187195881712No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG365870.38081758528530923No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA354330.3688061196439818No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC306000.31850160192774657No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC304520.3169611366635208No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA273660.28484035419459847No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT264540.27534775743126166No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA244930.25493659268027113No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC241590.2514601372866807No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC198710.20682827882046576No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA195430.20341427472137094No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG193380.20128052215943668No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT192390.2002500758002587No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG176360.18356517162084113No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT165230.17198045649190052No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA149380.15548290619596986No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG143550.14941472208081052No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT126000.13114771844083684No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG120880.12581854131054251No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG116080.12082243775089158No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA110800.11532672383527554No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA109800.11428586892701495No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG105160.10945630215268572No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA104310.1085715754806642No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT102830.1070311102164385No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA102700.1068957990783646No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT102610.10680212213662116No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC98980.10302381881963514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT459950.069.5446558
CAGCACG251200.069.45064
AGTAGGT463000.069.328057
TTGGCGA230800.069.2816317
TAGGTTG462200.069.244339
GTTGTAT480000.069.1919312
TAGTAGG463300.069.147916
TGTATAG602500.069.04204614
ATTGGCG249150.069.0391416
GAGGTAG917150.069.024712
TATAGTT537700.069.01800516
GGTAGTA827500.068.970474
GTAGTAG822050.068.938135
TGAGGTA891750.068.931881
AGGTTGT548100.068.8829310
AGCTTAT350500.068.825712
GCACGTA254950.068.772236
GTTGACA223800.068.7450818
TAGTTTG224400.068.605929
GTACAGG79000.068.5320410
Produced by FastQC (version 0.11.5)