FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH214.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH214.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10009620
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1271561.27033793490662No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG692950.6922840227700952No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA680260.6796062188174976No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG438490.4380685780279371No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT429800.4293869297735578No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG398920.3985366077833124No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC393390.39301192253052564No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA329160.3288436524063851No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC295340.2950561559779492No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC269950.26969055768350847No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT255730.2554842241763423No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG228000.2277808747984439No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA227900.22768097090598843No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC222890.22267578589397No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG170950.17078570415260522No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA168110.1679484336068702No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG166240.16608023081795312No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT158400.15824776564944523No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT153930.1537820616566863No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC151920.15177399341833156No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA116820.11670772716646585No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT116800.11668774638797477No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA111410.1113029265846256No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA109580.10947468535269071No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA105090.10498900058144066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT472700.069.409278
CAGCACG262950.069.2229164
AGTAGGT476100.069.200287
GTTGTAT488600.069.170812
TAGGTTG473750.069.144979
TAGTAGG475400.069.037856
GAGGTAG922100.069.027042
ATTGGCG257350.068.9473916
TTGGCGA239500.068.9424117
GGTAGTA829200.068.931274
AGCTTAT434250.068.890742
TGTATAG608400.068.8900714
TGAGGTA898150.068.875171
GTAGTAG825900.068.8592155
TATAGTT540900.068.8557716
TAGTTTG219300.068.829539
AGGTTGT564350.068.80385610
TGCTGGC118750.068.756729
GCTGGCA118600.068.7256410
GTTGACA281350.068.70325518
Produced by FastQC (version 0.11.5)