FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8402744
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1362191.621125194341277No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA596870.7103274834982477No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG584090.6951181661609588No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC390260.4644435198787444No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG386020.4593975491815531No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT369820.44011813283851087No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA346680.41257950974110363No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG323790.3853384085008421No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC316350.37648415803218566No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC276390.32892826438601486No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA256510.30526932630578774No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT250890.2985810349571521No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC227650.27092340311688656No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA185160.22035658827640114No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC180260.21452515987634516No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG175530.20889604633914827No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT171440.20402858875624438No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG170620.20305271706480646No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT169940.20224345761337012No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG165390.19682855981331812No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA135580.16135205356726326No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA117560.13990667810420024No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG106710.1269942295040763No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG106400.12662530240121558No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA105710.12580414207549342No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG101000.12019883028686819No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA98170.11683088286397873No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT97500.11603352428682821No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA96290.11459351849824297No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT96290.11459351849824297No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT93540.11132077806964011No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA92470.11004738452105646No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC91740.10917862069819097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT432100.069.517188
AGTAGGT435100.069.367657
GTTGTAT446350.069.2728712
TAGGTTG433400.069.2602169
CAGCACG227200.069.2402654
TAGTAGG434850.069.1910556
GAGGTAG850900.069.172452
TGTATAG559450.069.137214
GGTAGTA769550.069.080944
TATAGTT501200.069.0375916
ATTGGCG223600.068.9299116
TGAGGTA828450.068.9226151
GTAGTAG766700.068.877525
AGGTTGT512000.068.8740310
AGCTTAT355950.068.75622
TTGGCGA208400.068.73472617
ACAGTGG138900.068.7100454
GCACGTA229700.068.6855556
GTATAGT562400.068.625215
TAGTTTG206700.068.606669
Produced by FastQC (version 0.11.5)