FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH211.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH211.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7961069
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1375811.7281724351340253No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA633850.7961870447297968No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG550270.6912011439669723No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA526010.6607278494885549No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC461280.5794196734132062No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC445220.5592465032020197No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG442640.5560057323959885No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT404350.5079091765188821No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT374840.47084129028400584No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC326390.40998262921725714No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC318480.40004677763752583No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA302210.379609823756081No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA273990.34416232292422033No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG267240.3356835620944876No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT266490.3347414775578506No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG239750.3011530235449536No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC197600.24820787258595547No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG193820.2434597665213051No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA191560.24062095178423903No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG158230.19875471497609176No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT145340.18256342207309095No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG137740.1730169654351696No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC135150.16976363350198323No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT134690.16918582165284587No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA129980.16326953076276565No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG126930.15943838698044194No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT124570.15647396097182426No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA119770.15044461993734762No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA113930.14310892167873435No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC112910.14182768670890805No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG109360.13736848656882636No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG106450.1337131985666749No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA102160.12832447501711142No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA100470.1262016445278894No Hit
TGTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGT97490.12245842863565183No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA96430.1211269491572049No Hit
TGCAGATGATGTAAAAGAATATTTGCTATCTGAGAGATGGTGATGACATT92400.11606481491367555No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA91410.11482126332531473No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT89780.11277379959902371No Hit
CAAAATGATGAGATTCCACTTAATTGGTCCGTGTTTCTGAAACACATGAT89290.11215830436842088No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT89260.11212062098695541No Hit
TCAAATGATGAAATCACCCAAAATAGCTGGAATTACCGGCAGATTGTGTA89170.11200757084255895No Hit
GTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGTC87140.10945766203006155No Hit
TCAAAATGATGAGATTCCACTTAATTGGTCCGTGTTTCTGAAACACATGA84590.10625457460549582No Hit
TGCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAA84560.10621689122403033No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA80080.1005895062585188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT395050.069.294157
GTAGGTT393150.069.281878
CAGCACG198000.069.1994864
TTGGCGA180950.069.18169417
TAGTAGG394300.069.133936
TAGGTTG394150.069.079469
ATTGGCG194950.069.02460516
GTTGACA220850.068.9665818
GTAGTAG696600.068.95495
TGTATAG506350.068.9296114
GTTGTAT409100.068.92467512
GGTAGTA701650.068.912514
GCTGGCA90550.068.9118210
GAGGTAG779250.068.894542
AGCTTAT338950.068.786832
TAGTTTG193550.068.782539
TATAGTT455350.068.7335416
TGAGGTA755950.068.639071
GCTTATC339500.068.6032263
GCACGTA199900.068.5942846
Produced by FastQC (version 0.11.5)