FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH210.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH210.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8890878
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1753631.9723923778956365No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG571010.6422425321773619No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA542720.6104234025031049No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC510180.5738240925136978No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC413400.4649709511254119No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT332510.37399006037423976No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG329540.370649557895182No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG328490.36946857217026263No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA326570.36730905541612424No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA322870.36314748667117014No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC296140.3330829643596504No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT256410.288396714025319No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT238230.26794878975957154No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC235140.26447331748338015No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG217750.2449139443820959No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG215340.24220330095632853No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC214960.24177589659873863No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG184450.20745982567750904No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG166040.18675320930058875No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT163070.18341270682153102No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA155540.1749433520513947No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA148860.16743003334428838No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA138790.15610381786815655No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG126880.14270806550264215No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT123440.1388389313181443No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG108250.12175400449764354No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA96430.10845947947997936No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT95200.10707603905935949No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA90500.10178972200495834No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT353250.069.340138
AGTAGGT356350.069.178867
CAGCACG194050.069.147914
GTTGTAT370600.069.07812
TAGGTTG354650.069.056549
TTGGCGA176550.068.9865417
TGTATAG459750.068.952314
GAGGTAG702800.068.9053962
GGTAGTA630850.068.902684
TAGTAGG356300.068.86566
ATTGGCG191550.068.827816
GTAGTAG627550.068.7403955
TGAGGTA687650.068.73411
TATAGTT412050.068.706116
AGATGCT86550.068.6203466
GCTGGCA86550.068.61919410
AGGTTGT426150.068.51578510
AAGATGC86900.068.505075
GTTGACA182950.068.29687518
GCACGTA197200.068.255966
Produced by FastQC (version 0.11.5)