FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH209.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH209.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9278467
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1084241.168555107217604No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG616400.6643338818794097No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA580940.6261163616791438No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT366710.3952269270343905No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC350530.37778870151717947No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG350070.37729292996353814No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG348250.3753313990339137No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA332070.3578931735167027No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC281020.30287330870498325No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC271370.29247288372098534No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA249800.2692255089121942No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC221120.23831523030690308No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT207890.22405640931847903No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG189270.2039884390384748No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC189050.20375133090412456No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT161730.17430681167481654No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT160990.17350926613200218No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG159600.1720111738286077No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG151220.16297950943835873No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG146420.15780624105253593No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT138140.14888235308699163No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA136330.14693159979983764No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA128870.13889147851687136No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG114340.12323156400728698No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA110110.11867262124228065No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA95820.10327137015198738No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA94760.1021289400501182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT383600.069.419558
AGTAGGT387150.069.207877
GTTGTAT398100.069.1675912
TAGGTTG384750.069.148399
CAGCACG190650.069.076854
TAGTAGG386200.069.007316
TGTATAG493150.068.98659514
TGAGGTA726050.068.931661
GGTAGTA671300.068.8790364
TATAGTT444450.068.77566516
GAGGTAG750250.068.7398762
ATTGGCG188150.068.7127316
TTGGCGA173700.068.6667717
GTAGTAG669800.068.620885
GCACGTA192800.068.597356
AGGTTGT460900.068.5364210
AGCTTAT246750.068.505452
TAGTTTG178250.068.443259
CAGTGGC121450.068.352545
AGATGCT86800.068.301646
Produced by FastQC (version 0.11.5)