FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH208.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH208.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10227113
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC832920.8144233861501285No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG810400.7924034866926767No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA783120.7657292923232588No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT481930.4712278039755696No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG470300.4598560708188127No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG407160.39811821772185363No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA360260.3522597237363076No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC274240.26814996568435295No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC262710.2568760118324692No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT242370.23698770122125373No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC216710.21189753159078228No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC213100.20836769868485855No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC201190.19672218347445658No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA176240.17232624690858506No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA174650.17077155596110064No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT169400.16563814245525596No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG162290.15868603387876912No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG130680.1277779956083403No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA130440.12754332527664455No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA126110.12330948137563356No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG118130.11550669284674962No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT117310.11470490254678911No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG113720.11119462550184006No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG113590.11106751240550486No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG113570.1110479565445302No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT111480.10900436907267964No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA103540.10124069226574497No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT552250.069.3928158
CAGCACG306100.069.321644
AGTAGGT556150.069.296697
GTTGTAT568050.069.2373912
TAGGTTG553300.069.210869
GAGGTAG1087850.069.150442
TTGGCGA279700.069.0956417
TGTATAG716400.069.0575714
TAGTAGG555750.069.057566
TGAGGTA1061850.069.0015951
TATAGTT638300.068.9763716
GTAGTAG982250.068.9593665
ATTGGCG301950.068.9530616
GCTGGCA120550.068.9500610
GGTAGTA989000.068.923984
AGGTTGT652750.068.8156510
AAGATGC121100.068.782425
AGATGCT121100.068.724276
GCACGTA309100.068.716436
TGCAGGT94750.068.6661415
Produced by FastQC (version 0.11.5)