FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH205.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH205.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10163981
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC911220.8965187951453273No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG719840.7082264321430746No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA705080.6937045632021547No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG452580.4452782822006456No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT440670.43356043266904964No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG437290.4302349640362374No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA302710.2978262159285815No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC253080.24899692354796807No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC247120.24313307944987306No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT220710.2171491662568043No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC209840.20645453784299675No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC201180.19793425430448952No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA173530.17073034670175002No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC169790.16705068614354945No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA161980.15936668909554239No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG146860.1444906282292342No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA144960.14262128195635154No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT137420.1352029288523857No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA130530.12842408894703758No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT127420.12536426425826652No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG125480.1234555633270074No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG113850.1120131964040468No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG112670.11085223398194075No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA108280.1065330602251224No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG105150.10345355820716312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT568450.069.464188
AGTAGGT572350.069.333267
GTTGTAT592650.069.3030812
TAGGTTG569800.069.238189
TAGTAGG571150.069.2283866
CAGCACG291600.069.190214
TGTATAG750450.069.1415914
GAGGTAG1122600.069.124542
TGAGGTA1093950.069.073561
TATAGTT668950.069.0072416
GTAGTAG1010050.068.968725
AGGTTGT676250.068.93239610
GGTAGTA1018000.068.822034
AAGATGC117000.068.738025
GTATAGT751750.068.7154315
GCTGGCA117100.068.708210
TTGGCGA265200.068.703117
TTGTATA754100.068.667713
CGTAAAT294700.068.651719
TAGTTTG266700.068.6158149
Produced by FastQC (version 0.11.5)