FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH204.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH204.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8664750
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG601070.6936957211691047No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA585310.6755070832972676No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC545530.6295969300903084No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG363170.41913500100983875No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT355640.4104446175596526No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG318830.3679621454744799No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA255980.29542687325081507No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC222950.2573069044115525No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT209270.24151879742635393No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC163980.18924954557257856No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC153360.17699298883406905No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC148230.17107244871461957No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC131600.15187974263539053No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG128790.1486367177356531No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA123360.1423699471998615No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT111990.12924781442049685No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA103570.11953028073516257No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG99070.11433682449003145No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG91600.10571568712311377No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA89090.1028188926397184No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT460950.069.3794868
AGTAGGT463900.069.330597
TAGGTTG462050.069.146149
CAGCACG238200.069.1276254
TGTATAG583250.069.1244114
GAGGTAG883100.069.0275042
TAGTAGG464150.068.999186
AGGTTGT538500.068.9741310
GTAGTAG797800.068.950435
GGTAGTA803250.068.8834464
TATAGTT520700.068.8803416
GTTGTAT472700.068.8765212
TTGGCGA216150.068.86307517
TGAGGTA861150.068.862911
ATTGGCG234100.068.8299916
AGCTTAT362200.068.767352
GCTTATC362200.068.6997153
TGCAGGT74150.068.58012415
AGATGCT97750.068.562366
CGTAAAT241450.068.545019
Produced by FastQC (version 0.11.5)