FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8521638
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC749720.8797839100886473No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG610150.7160008439691994No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA573800.6733447255093445No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT355350.4169972955903548No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG348610.4090880180547448No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG344870.40469919046080105No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA283120.33223659582817294No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC233230.27369151329826497No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT215570.25296779797499025No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC207390.24336870446738057No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC188760.22150671032963384No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC183600.21545153642996803No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA157890.1852812804298892No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC155080.1819837923178619No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG149590.17554136892461283No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA145490.17073008733766912No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG143480.16837138587675282No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT122170.1433644564577843No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA117810.1382480692092295No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG115670.13573681491750764No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA110300.12943520952192525No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA110270.1294000050225086No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG106130.1245417841030093No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT100680.1181463000423158No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG98340.11540034908781621No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG89110.10456909810062338No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA86540.10155324598392938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT461900.069.410178
AGTAGGT465250.069.2944267
TAGTAGG464100.069.203426
TGTATAG604350.069.19485514
GAGGTAG917400.069.182252
GTTGTAT479300.069.175812
CAGCACG235650.069.1635364
TAGGTTG463550.069.1404659
TGAGGTA885500.069.022041
GTAGTAG825500.069.020125
GGTAGTA832550.068.9443054
AGCTTAT415350.068.9331362
TATAGTT544350.068.9279716
AGATGCT107950.068.892946
AGGTTGT545350.068.8829910
GTTGACA263250.068.7749618
GCTTATC416000.068.766943
AAGATGC108250.068.766675
ATTGGCG232450.068.68535616
GCACGTA238300.068.658766
Produced by FastQC (version 0.11.5)