FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH202.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH202.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10466283
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG743070.7099655149779535No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA741040.7080259534354268No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC696340.6653173815384124No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG476790.4555485457444634No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT458150.4377389757185049No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG450010.42996162056768383No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA330770.3160338775475496No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC277930.2655479504997142No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT234740.2242821066466481No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC228640.218453867528711No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC193720.18508958720111046No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC188970.18055120428140536No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC174950.16715580880050732No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA161010.15383684924246746No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG159430.15232723976601817No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT154920.14801816461488762No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA150440.14373775293482893No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA139320.1331131596575403No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA111790.10680964770396521No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG109510.10463122390250675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT564250.069.448998
AGTAGGT567900.069.372397
GTTGTAT589200.069.3120612
GAGGTAG1106300.069.1947862
TGTATAG738450.069.1762414
CAGCACG298100.069.16164
TAGGTTG565900.069.160259
TAGTAGG566400.069.1489646
TGAGGTA1076450.069.0881651
TATAGTT659750.068.93552416
AGGTTGT673750.068.93548610
GTAGTAG992150.068.909035
GGTAGTA999750.068.818934
CAGTGGC195050.068.775365
TTGTATA742600.068.737813
GCACGTA300150.068.701216
GCTGGCA118150.068.66218610
TGCTGGC118100.068.631999
GTATAGT741000.068.62710615
ACAGTGG194950.068.612834
Produced by FastQC (version 0.11.5)