FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH201.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH201.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8692051
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC811280.9333585364374876No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG613400.7057022560037901No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA606380.6976259113067791No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG390770.44957168336909203No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT378630.4356048992349447No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG368020.4233983440732228No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA243630.28029057813857744No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC230440.26511579372923605No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC212630.24462580810904125No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT200750.2309581478525609No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC194430.2236871366723458No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC176920.20354229398792067No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC163130.18767722370703993No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA154820.1781167643862191No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT154170.17736895469205138No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG127670.1468813286990608No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA126770.14584589989175167No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT123170.14170418466251522No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA118330.1361358786320973No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA99850.11487507378868347No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG95300.10964040592950962No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG93650.10774211978277624No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG93020.10701731961765987No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA87210.10033305142825323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT499400.069.4068768
AGTAGGT502800.069.3690957
GTTGTAT514350.069.3351412
TAGGTTG501050.069.150379
TGTATAG642200.069.145414
TAGTAGG502650.069.125626
CAGCACG258550.069.102164
GAGGTAG961900.069.093232
TATAGTT573900.069.0015316
TGAGGTA936750.068.989421
AGGTTGT587550.068.9233610
TTGGCGA234650.068.9071617
GGTAGTA875400.068.896124
GTAGTAG869850.068.8734055
ATTGGCG252500.068.8451216
TTGTATA645100.068.78030413
GCACGTA260550.068.6661456
GTATAGT644150.068.6644215
TAGTTTG225700.068.6629
AGCTTAT330050.068.5322342
Produced by FastQC (version 0.11.5)