FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl29-bzip8-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl29-bzip8-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4620301
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77690.16814921798384996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG4104.474714E-811.9543621
CGGGGGT5501.6189006E-1011.4575581
CGGGGAG9000.011.2807441
CCCGTCC2802.3413087E-411.2529571
CGCGGGG3451.1574939E-511.1623541
GCGCGCC8950.010.94963662
CCGGGGT2650.001867577710.5688151
GCCGTAT7051.0913936E-1110.4282731
CGGGGTG15950.010.31351113
CGAGCGC9700.010.10301559
GGGGTAT3551.8933954E-49.8617471
GGCGCAC4804.364152E-69.47909465
GGGGCAT6301.8273568E-89.4469271
CGGGGAT25200.09.4469271
CCCAGGC17150.09.38747967
GGCGTAT5603.2019125E-79.3774641
GCGGGAT13100.09.3536031
CGGGCGC3000.0052277659.33336315
GTCGGGC3400.00139794539.2671411
GGGGTGC17400.09.25290214
Produced by FastQC (version 0.11.2)