FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl29-bzip8-09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl29-bzip8-09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5481694
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT126840.23138832630934889No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA122130.22279609186503296No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG106420.19413706784800464No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG106220.1937722171285008No Hit
GCAGAATTCACCAAGTGTTGGATTGTTCACCCACCAATAGGGAACGTGAG86410.15763375336164331No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84660.15444130956598454No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT71270.130014553895201No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC67340.12284523725695014No Hit
GTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCTTAATTGGCCGG66690.12165947241856258No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC66010.12041897997224947No Hit
GAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGC65590.11965279346129135No Hit
GTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG65490.11947036810153941No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA64410.11750017421621856No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA62760.11449015578031171No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGA60770.11085989112124828No Hit
CGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTG59610.1087437569481259No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTT57560.1050040370732113No Hit
GGCCGGTCCGCCTTTGGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCG56030.10221292906900678No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT54920.10018800757576035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGAC8600.030.5309851
CCCTACA2400.029.16580410
CAGACGT9500.026.894433
CGGGGAC3400.026.7714841
GCCAGTA16200.026.1485961
CAGTAGT16500.025.6663763
CCGGCAT4550.023.8521921
AGACGTG11050.023.7553864
GGATATC8850.022.9375932
CCAGTAG18250.022.821662
TAGTCAT18500.022.7024466
CGGCCAG11950.021.96647815
CGCGGTT5600.021.880541
GCGGGCC1957.7852746E-1021.5439171
CATATGC19800.021.0347310
GTCATAT20200.020.7912758
TATCTCG9650.020.6733425
GCAGACG12600.020.5553232
CCCCAGT2051.4533725E-920.4929941
CGCGTTC3100.020.322353
Produced by FastQC (version 0.11.2)