FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl29-bzip8-08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl29-bzip8-08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4130421
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58350.14126889244462004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA14250.024.07243520
GGGCGGG5350.020.9345192
CGCGGGG3450.018.2650171
GGGGGAG19400.018.0431521
AGAGGGG10000.017.50100726
AGGGGGG12650.014.66469928
GAGGGGG13300.014.21134427
GTATGCC4051.8553692E-1013.82661747
CCGGGGT3102.3822759E-713.5514641
CGGCAAT8800.013.5257991
AAGGGGG5500.013.36359656
GCACACG6050.013.30542411
CGGGCGC5103.6379788E-1213.03933515
TATGCCG4851.8189894E-1112.989181548
CGGGGGG7700.012.7272355
AGTGCGC2000.002783578812.24996464
CGTATGC4953.43789E-1012.0197346
CGAGCGC9650.011.96902259
GGGAGGG15350.011.8566422
TGGGGGG6550.011.7556925
Produced by FastQC (version 0.11.2)