FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl29-bzip8-07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl29-bzip8-07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3332743
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46970.14093495958134186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4200.025.8321548
GTATGCC4650.023.33226447
CGTATGC5000.021.69900546
GCACACG6250.019.03998411
ACGTCTG6650.017.89525815
CTCGTAT6300.017.22143244
CCGGGGT2852.6739144E-1017.1960641
ATGCCGT6800.016.9847749
TCGTATG6750.016.59183145
GTCCCCC2452.4829205E-715.713869
AGTCACG7300.014.86478528
CACACGT8900.013.76403312
CGGGGGG3701.0682925E-813.2456171
AGCACAC8800.013.12498910
ACACGTC8850.013.05083713
CACGTCT9450.012.96295214
CGGGACT5750.012.78491
TACGGGC2203.9341388E-412.72668847
GCCCGCA3902.2379027E-812.56352748
GTCACGT9300.012.42027629
Produced by FastQC (version 0.11.2)