FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl29-bzip8-06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl29-bzip8-06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4378767
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60570.13832661112134992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT2450.027.14691
GTATGCC7100.021.68965747
TATGCCG7400.020.33738348
GCACACG8200.020.06029911
GGCCTTA8300.019.39715636
CGTATGC8200.019.20688646
ACGTCTG9550.018.32482715
GCCTTAT9750.018.30728337
AGTCACG9550.016.85903228
ATGCCGT9600.016.40588449
AGCACAC10950.016.3008210
CTCGTAT9550.015.39232644
GTCACGT10900.015.09192429
TCACGTG11450.014.67249730
CACACGT12200.014.63065312
CGGGCGC6350.014.33087915
TATCTCG11400.014.12249241
CACGTCT12400.013.83033614
TCGTATG11200.013.74969345
CGGCAAT10550.012.9403161
Produced by FastQC (version 0.11.2)