FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl29-bzip8-05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl29-bzip8-05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4348333
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63740.14658490966538212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCC2151.9739053E-514.6543351
GCGGACG2701.1669888E-512.9626392
GCGGTAT4355.7298166E-1012.8763511
GGGGTAT3751.8138417E-712.1359621
CCCTACA4903.750756E-911.42828710
CCGGGAT9850.011.3730211
CGGGCGC4351.0205804E-711.26460515
CGGCAAT10600.011.2288471
CGCGCGT4451.3958925E-711.01235420
CGCGGAC2250.00669489610.8912471
GTCGAAT14650.010.75318051
GCGGGGC3652.1609563E-510.5502291
GTAGCGC5906.2700565E-910.0851916
AAGGGCC7001.0004442E-109.99975152
GGCGCAC7051.1459633E-109.92882965
GCGCACC7051.1459633E-109.92871666
CGGGGAG11650.09.916311
CGGGGGT5653.439709E-89.91365051
GGATAAC34900.09.8302091
GCGGGAT13250.09.77570151
Produced by FastQC (version 0.11.2)