FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl29-bzip8-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl29-bzip8-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4258244
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63150.1483005670882176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC5050.026.33595833
TATGCCG6700.020.37261246
GTATGCC7000.018.99951445
GCACACG8100.016.8514211
ATGCCGT8750.015.1996147
CACCCGT8750.014.79979431
CTCGTAT9000.014.777225542
TCACCCG8900.014.55035930
TCGTATG9900.013.43384143
ACGTCTG10400.013.12527815
CGGGCGC4304.783942E-1013.02353315
CCCTACA5357.2759576E-1212.42958710
CCGTCCA11000.012.40877334
GGGGGGC2601.177706E-412.11552
CACACGT11550.011.81787912
AGCACAC11750.011.61672310
GCGGGAT13800.011.4161011
CGCGTAC2802.3411152E-411.2530151
GACGCGC5351.2496457E-911.12147156
TGCCGTC13050.010.99588748
Produced by FastQC (version 0.11.2)