FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl29-bzip8-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl29-bzip8-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4420578
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72210.16334967961203262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG2957.792551E-915.4277081
CGGGCGC3653.45608E-1115.34243215
GCGGGCC1400.003749734715.0038691
GCGGGGC2405.5570134E-513.1283861
CGGGGGT4802.055458E-1012.3990311
GCATCCC13800.012.17388564
GCTACGT13600.011.83780853
TACGTGG13900.011.83423755
GGCATCC13050.011.8007463
GGGGTAT3903.0168667E-711.6696771
CCCTACA5551.9099389E-1011.35094110
GCGATTT19450.011.3396851
TGCCCCC4954.3910404E-911.31400119
CGAGCGC3104.8068192E-511.2899148
ATAGGGG4351.0207623E-711.2644761
CCGGGAT10700.011.1243641
GGGGCGA4105.781603E-711.09753664
CGGGGAG11300.010.8435041
TAGCCCG5209.50422E-910.76884234
TAACTAG15000.010.732944547
Produced by FastQC (version 0.11.2)