FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl29-bzip8-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl29-bzip8-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5161293
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77740.1506211718652671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA6800.016.9851110
CGGGGGG4506.91216E-1113.2246411
CGCGGGG3801.5525075E-812.8970961
GGGCGCT2254.7464966E-412.446721
TCCCTAC8750.012.3998649
GTATGCC6700.011.49207847
CGGGGAC5259.113137E-1011.3354061
TATGCCG6209.094947E-1211.2898748
CGGGTAG3551.5902258E-510.8470541
GGGGACC3551.5902258E-510.8470541
GGCGCGC5653.081368E-910.53055368
TACACGA11450.010.39300113
CTGGGGG2700.00218324410.3722671
CCGGGGT4501.909888E-610.112961
CCTACGC11500.010.04317457
CTACACG9150.09.94524712
GTCGGGC3602.181643E-49.7241
CCTACAC12650.09.68368911
GCGGAAC11950.09.6670391
CGGCAAT11300.09.6035261
Produced by FastQC (version 0.11.2)