FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl29-bzip8-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl29-bzip8-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4769513
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74500.15620043388077567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA5600.016.8745210
CGCGGGG2854.944013E-915.9674721
TCCCTAC7150.013.7059039
TATGCCG6200.013.5478648
GTATGCC6200.013.5478647
GGGGTAT4357.872586E-912.0708991
GGCGCGC6055.456968E-1211.57004168
GCACACG8600.010.9880611
CCGGGAT12500.010.9217511
GGCAGAC7201.8189894E-1210.6961581
GGGGCAC3954.446725E-610.6346161
CCGGGGT4106.938899E-610.2455441
GCCGGAT11800.010.0863621
GCGGGGG8350.010.0614931
CACGAGT26850.010.03706568
ACGTCTG9800.09.9998215
GGGGAAC7453.092282E-119.8673531
CGCCTAT2850.00341887459.8261371
CTACACG9750.09.69213412
GGCCATC27900.09.65922310
Produced by FastQC (version 0.11.2)