FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl28-bzip11-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl28-bzip11-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5392261
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85270.15813403690956354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG4700.018.6208131
CCGGGGT5500.015.912331
CGGGGGG7850.014.71637251
GCGGGGG9900.014.4979011
CCCCCCT1950.00229866112.5666611
GCGGGGC4852.4374458E-1012.270541
GGGCCCT2651.4041453E-411.8892131
GCGCGCC18300.011.85769862
CCTACGC7450.011.74474357
GGGCGCT3601.4591988E-611.6690431
CGGGCAT7250.011.5885671
GGGGTGT5005.131369E-911.2022811
GCGGGAT14100.010.9242111
GCCGGGG14250.010.8092181
CGAGCGC19650.010.68701859
CCCTACA6251.3460522E-1010.639710
CGGGGAC6603.274181E-1110.608221
CGGGGAG9600.010.575071
CGGCAGT6704.3655746E-1110.4498891
GCGTATT9750.010.0511852
Produced by FastQC (version 0.11.2)