FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl28-bzip11-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl28-bzip11-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5955437
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90980.1527679664817208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG4900.020.7193951
CGGGCAT8400.015.8372391
CCGGGGT5600.015.6288541
CGGGCCC2759.180676E-713.9996912
GCGGGGG12300.013.6619061
CCCTACA7200.013.124269510
TCCCTAC8700.012.8728479
CGGGGAG13850.012.6384951
CGGGGGT9550.012.4638071
CGGGGGG9450.012.2252371
GGCCGAT6150.011.9541681
GCCGGGT18650.011.2628321
GTCCGCT3752.3834345E-611.2027631
GGGCGAT5955.4569682E-1111.1792271
CGGGGAC6950.011.081871
GGGCCCT3551.5892161E-510.8477461
GGGGGGC7251.8189894E-1210.6204553
GGGGAGC11900.010.5908471
GGGGTAT4301.0648855E-610.5840051
GGGGCCC3004.4339633E-410.5025891
Produced by FastQC (version 0.11.2)