FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl28-bzip11-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl28-bzip11-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5611411
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101100.1801685886134521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT6200.019.7617841
GCGGGCC3600.017.5032941
CGCGGGG3950.015.9523691
CCCTACA6200.014.67691110
GCCCGAC3507.382005E-813.0024471
CCGGGAT11950.012.8894541
CGGGTGT4902.1827873E-1112.8595631
CGGGGAG12250.012.5737941
GCGGGGG11700.012.2672651
CGCCTAT3205.0160797E-612.0335131
GGGGGTA3501.0414678E-611.9999064
GCCGAGT10350.011.8379761
CGGGCAT7000.011.5021651
CGGGCCC2751.98217E-411.45671
GCCGGAT14500.011.3469621
TAACGTA21600.011.3425034
ACGTAAT23250.010.9890636
GCGGGTT8300.010.9659191
GGGGGGC4501.6315789E-710.88880353
CGAGCGC24100.010.74680259
Produced by FastQC (version 0.11.2)