FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl28-bzip11-09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl28-bzip11-09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8960801
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133920.14945092520188766No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC90270.10073876208164873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT8550.018.8338721
CGGGGGG12150.017.8633671
CGGGCAT12150.015.5584151
CCCTACA10250.015.02379910
GGGGCAC7000.013.5024821
CGCGGGG7550.013.446181
GCGGGGG17900.012.12513351
CGAGCGC37550.012.024359
CGGGGAG19000.011.7916411
GCGCGCC38500.011.6366262
CGCGGAC4257.381823E-811.531531
CGGGCCC3702.0354619E-611.3510312
GGGGGGC6800.011.323212
GCGGGCC5209.478754E-910.7712111
GCCAAAT83450.010.7389421
CGGGTGT9800.010.7162551
CCGGGAT21800.010.5982781
TCCCTAC13000.010.4995869
ACGTAAT35200.010.4400466
AACGTAA39000.010.4099635
Produced by FastQC (version 0.11.2)