FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl28-bzip11-08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl28-bzip11-08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5215007
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95050.1822624590916177No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC53240.10208998760691979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG4000.018.3789481
CCGGGGT4350.017.7049521
CGGGGGG7450.016.9163861
CGGGCGC6700.014.10493815
CGGCAAT10800.013.6140361
TAGCCCG6750.013.48088734
CCCTACA5750.013.39071310
CGGGGAG11300.013.3214451
GCGGGCC2659.602698E-613.2103841
TCGGGCG6750.012.9623927
CGCGGAC2508.149285E-512.6027071
CGGGCCC2305.7097577E-412.1765281
CGGGTGT5551.4551915E-1111.9845551
CGGGCAT7750.011.7444591
GGGGCAT7500.011.6691731
GGCGGGC7800.011.66682413
ATCGGGC7500.011.6663766
GCCGAGT10250.011.2707141
CCCGGGT8750.011.2024061
GCGGGCG8850.011.07359514
Produced by FastQC (version 0.11.2)