FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl28-bzip11-07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl28-bzip11-07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5186840
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88080.16981437638330854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT4950.015.5590551
CGCGGGG4100.015.369311
GCGGGGG7950.014.9719211
CGGGGGG6100.014.3474891
CCGGGAT13100.013.89625551
GGGGTAT3902.2306267E-812.5669291
CGGCAAT9600.012.3986221
CGGGCAT6250.012.3227721
TAACGTA17800.011.6008254
GCCGGAT12850.010.8973921
GCCGAGT9350.010.8580571
GTCGGGC3257.753945E-510.7716531
CGTAATT20700.010.6517977
CTGGGGT6957.2759576E-1210.5779181
AACGTAA22550.010.5540525
CCCTACA6402.0736479E-1010.39035710
ACGTAAT21250.010.3762036
CTGGGGG3055.164701E-410.3301931
GGGCGAT5159.4592906E-810.1964671
GGGGCAT5906.2536856E-910.0870141
Produced by FastQC (version 0.11.2)