FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl28-bzip11-06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl28-bzip11-06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6157369
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102070.16576885354767595No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG99490.16157875222355522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGG10050.016.0233541
CCGGGGT5650.014.8704621
CGCGGGG4950.014.85168651
CGGGGGG8250.014.4273521
GGGGTAT6950.014.1037591
GGCGCGC4951.8189894E-1213.43396668
CCCTACA7150.012.72701810
CGGGTGT5900.012.4603121
GGATAAC59400.012.3764061
CGGGGAG13250.012.15356351
GCGGGAG15200.011.7459521
CGGGGAC6107.2759576E-1211.4778841
GGGCGCT3054.0623665E-511.4778841
GCGGGAT16000.011.1586551
CGGGCAT7300.011.0297751
GGCCGAT6401.8189894E-1110.9398581
ACATGGC68450.010.58441456
CGGCAAT12300.010.53072551
GGGAAAT30950.010.5192311
TAACATG71050.010.4927434
Produced by FastQC (version 0.11.2)