FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl28-bzip11-05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl28-bzip11-05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5527635
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94710.17133909890938892No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG70510.12755907363637434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG5050.015.25141051
CCGGGGT5750.015.221271
GCCGGAT13150.013.5776041
CGGGTGT4659.094947E-1213.5518411
GGGGAAT20000.012.6032111
GCGGGCC2508.146404E-512.6032111
AATAGGG7550.012.05256236
ACATGGC55100.012.0052466
GGATAAC57700.011.95278551
ATGGCCA55450.011.73998
CACGAGT55500.011.72942968
TAACATG55250.011.7193694
GGCCATC54450.011.69861210
ATGCGCT56700.011.41966629
CCCTACA6451.8189894E-1211.3951610
GATAACA58100.011.3252162
ATAACAT58800.011.2499163
TGGCCAT57600.011.2411349
GCCACGA59350.011.0274266
CGCGTAT8600.010.9911731
Produced by FastQC (version 0.11.2)