FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl28-bzip11-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl28-bzip11-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5795074
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100170.17285370298981514No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG64580.1114394742845389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG4650.020.328781
GCGGGCC2602.7604983E-816.1587721
CGGCAAT17450.014.2450291
CCGGGGT4202.1827873E-1114.1709861
CGGGGAG17400.014.0847521
TCCCTAC7050.013.4037159
CGCTATG15200.013.12526329
CCCTACA5900.013.05043410
AATAGGG10750.013.02273236
CGGGCGC15400.012.72730815
TCGGGCG18400.012.5538427
GGCAATC21750.012.3907332
GTCCCCC10200.012.35265669
GGCGGGC16050.012.21155713
CGGGGAC6050.012.1524651
TAAGCGG15000.012.13410120
TAGCCCG17400.011.86733734
GGCGCGC9550.011.72737768
GGCTGCC17350.011.69981743
CGGGGGG8800.011.5376081
Produced by FastQC (version 0.11.2)