FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl28-bzip11-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl28-bzip11-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5200570
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74900.14402267443760972No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG65430.12581313202206681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT4300.019.539811
CGCGGGG3655.7298166E-1014.3871881
GGGGTAT4152.7102942E-1013.4973771
CGGGGAG12300.012.5234811
CGCGCGT5053.6379788E-1112.4783931
GCAAGGG21400.012.2694471
CGGGAAT11450.012.2301561
CGGGGAC5451.382432E-1011.5625481
CGGGCAT7000.011.5028991
GTCGGGC3702.028075E-611.3542131
ATCGGGC9400.011.170026
GGGGAAC7400.010.8811211
CGGGCGC7151.8189894E-1210.76935715
CGGGGGG6201.1459633E-1010.7285111
TCGGGCG8700.010.4592897
GCGGGGC3351.05697836E-410.4475822
GCGGAAT9400.010.4281611
GGATAAC50800.010.2683361
GCGGGAG13300.010.2657451
GCGGTAT5502.2657332E-810.1843851
Produced by FastQC (version 0.11.2)