FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl28-bzip11-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl28-bzip11-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6015375
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102250.1699810901232259No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG82000.136317353448455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG4800.016.7746851
CGGGGGG8650.015.3792541
GCGGGCC3009.735231E-915.1701511
CCGGGGT5600.015.0034451
CGGGGGT7100.014.2990591
GCGGGCT6150.013.66167451
GCGGGAT10450.012.0601861
GGGCGCT3255.9605118E-611.8488751
GCAAGGG25600.011.6237631
CGGGGAG10850.011.6155711
GCCGGGT9250.011.3539581
GGGGCCT5602.2191671E-1011.25258351
CGGGCAT6251.0913936E-1111.2025731
GCGGGGG8200.011.1001111
GGGGCAT6855.456968E-1210.7323921
CAAGGGC26350.010.6261532
CGGGAAT14600.010.5503671
GGGGAAC8450.010.3574081
GGATAAC57750.010.2447761
GGTACCT31450.010.23920721
Produced by FastQC (version 0.11.2)