FastQCFastQC Report
Mon 18 Jul 2016
H7JJTBGXY_n01_sl28-bzip11-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJTBGXY_n01_sl28-bzip11-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6330573
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96590.1525770258079324No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG80080.12649723808571514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGC5150.018.3534151
CGCGGGG5200.016.1572781
CCCTACA7800.014.35839710
GCGGGGG14300.012.485171
CGGGGGG8650.012.1413071
GTCCCCC6400.012.03076669
GGGGCAC5601.8189894E-1111.8775231
CCGGCAT10200.011.6691471
CGGGCAT10250.011.6122241
GGGGTAT6351.8189894E-1211.5772631
GTCGAAT20900.011.3899791
GGGCGCT4105.767106E-711.0999181
CCGGGGT6653.6379788E-1211.054981
GCCAAAT49850.010.9551861
GCAAGGG26250.010.9356571
GGCCGAT6151.0004442E-1010.8153061
ATAGGGG6805.456968E-1210.80881561
GGATAAC68500.010.6299951
CCGGGAT14600.010.5501871
GGGGCGA7650.010.52278464
Produced by FastQC (version 0.11.2)