FastQCFastQC Report
Thu 21 Jul 2016
H7JJJBGXY_n01_bgm42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJJBGXY_n01_bgm42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8479152
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG756730.8924595289717652No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG169780.20023228737968135No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG128660.15173687180038758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG142300.031.7888431
AGGGGGG51950.021.1839471
CGTGAAA8200.015.64702133
ACACGTC9500.014.68147813
TAGTTAC16150.014.0685059
GAAACGA8900.013.65687636
TGAAACG9100.013.60455335
TCGTATG10300.013.10466745
AACGATC9650.012.35900438
AAACGAT10450.012.275837
ACGTCTG10950.012.12124515
ACGTGAA10650.012.0473332
CGCATAA8700.011.6357662
GTCCGCA9100.011.6235831
CTCGTAT10550.011.51464844
CTTAGTT20750.011.3833567
CACTTAG19400.011.3637985
ACTTAGT20400.011.3581126
GTGCATT18650.011.222451
TAATTGG19350.011.1647327
Produced by FastQC (version 0.11.2)