FastQCFastQC Report
Thu 21 Jul 2016
H7JJJBGXY_n01_bgm41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJJBGXY_n01_bgm41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11914318
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAG776520.6517536295405243No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAA339260.28474982789614983No Hit
GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG212950.17873452764984116No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT212540.17839040388211896No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA185580.15576216783872984No Hit
GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG158230.13280659455287327No Hit
GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGT145560.12217233080399567No Hit
GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT139380.11698529450028108No Hit
GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGAT123760.10387501827632936No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGT121300.10181027566999638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATG165400.023.2979662
GCCTTAT175300.022.209231
TTACGGC16250.021.04355640
TATGTGA186500.020.7945295
CTTATGT186600.020.4699463
CGTACGG5500.020.04323223
ATGTGAT196350.019.923216
ATTACGG18550.019.8916539
TAGTTAC54600.019.8584379
TAGATGC199050.019.5966212
GATAGAT200050.019.57721110
TGTGATA200450.019.5718147
GTGATAG201850.019.4026328
ACTTAGT57250.019.1749826
GCCTCAC60650.018.8496021
CACTTAG57900.018.8043125
ATGCCTC207850.018.73517415
TCACTTA61100.018.2244594
ATAGATG216350.018.19590411
CTTAGTT63000.018.0676087
Produced by FastQC (version 0.11.2)