FastQCFastQC Report
Thu 21 Jul 2016
H7JJJBGXY_n01_bgm40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJJBGXY_n01_bgm40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12558319
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGC17500.019.54127340
ATTACGG18950.019.3533839
ACCCGTC10200.018.75833932
CGTCCCG10200.018.09613635
GCCTTAT39350.017.2146741
CCTTATG35400.016.967412
CTTATGT38950.015.18956853
TGTCGGA23100.015.09684124
GACCGCG10000.014.18130432
GTCGTTT26350.014.1776581
ATTGCGT11850.013.85814645
TTATTCG18350.013.61022725
TGTGTCG26050.013.55925322
GCCGTAT12150.013.52146151
TATGTGA43200.013.4348595
AGTTACG19600.013.4284845
CGTATGA10750.013.39773127
TAATTAC50500.013.28069937
TCACCCG20900.013.03138330
ATCGTAT12500.012.95987825
Produced by FastQC (version 0.11.2)