FastQCFastQC Report
Thu 21 Jul 2016
H7JJJBGXY_n01_bgm39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJJBGXY_n01_bgm39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12281411
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158390.12896726605762157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAT11400.020.13764237
CCGTATC11750.017.81104538
ACACGTC15100.015.49359313
CGTCTGA17000.014.15997216
TATCGCG1758.199586E-714.14095341
GATCGGA16750.013.9708841
ACGTCTG18150.013.51030315
TAGTTAC18750.013.0773889
TGGGGGG72650.012.8843671
AGTTCCG18050.012.72094634
TTCCGTA19000.012.4398336
TACCGCG6200.012.337222
GTTCCGT19550.011.62967335
GTCGCAA5100.011.4711921
CTCGTAT15700.011.46281844
CACGTCT22200.011.14642114
CACTTAG22300.011.0964385
ACTTAGT25950.011.0960486
GTCACAT55350.011.0168411
TTAGTTA26350.011.01297958
Produced by FastQC (version 0.11.2)