FastQCFastQC Report
Thu 21 Jul 2016
H7JJJBGXY_n01_bgm38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJJBGXY_n01_bgm38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13908338
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG206970.14881001597746618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTAC40500.016.9411359
ACTTAGT44750.015.43279656
CACTTAG43550.015.0832185
TCACTTA44300.014.8786394
CTTAGTT47800.014.6833277
TTAGTTA61950.014.1618848
ACACGTC17750.013.9409913
TGGGGGG97850.013.5923041
AGTTACA53900.013.27199210
TATGGAT53250.012.97079121
TTATGGA53850.012.86800320
GATCGGA19250.012.6258141
TTACAGT55250.012.62201612
ACGTCTG20350.012.6022815
TACAGTT55800.012.53792113
TCGTATG19900.012.434937545
TTTTATG56450.012.35498818
ATGGATA57300.012.21088822
CTCGTAT18950.012.10867244
TTTATGG57750.011.96003719
Produced by FastQC (version 0.11.2)