FastQCFastQC Report
Thu 21 Jul 2016
H7JJJBGXY_n01_bgm37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJJBGXY_n01_bgm37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14429356
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG316220.21915045966015395No Hit
GGCAGGTTGACATAAGAGGCCTCTGTGAGTGGCTGATGGTCAGCCCTGGGA157860.10940197192445733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGC12350.016.57693740
GTCCGGT12300.016.4670681
TGGGGGG124300.015.4257861
ATTACGG14250.015.15763239
CCCGTAT11300.015.1361011
GACATAA56850.015.11589
GTTCGCT12750.015.0033271
ATAAGAG56300.014.98382212
GCCGTAT11550.014.808481
GTCGGGT16250.014.5416871
TAATCCG8850.013.98266225
CATAAGA60450.013.91794211
TGACATA65650.013.4665418
TAAGAGG62800.013.39712813
GCCTTAT33100.013.394211
ACATAAG67250.013.2799510
GCCCGTT25100.013.0905531
CGTCCGA7050.013.0848839
CCTTATG18550.012.9759632
GTCGTTT18550.012.8599961
Produced by FastQC (version 0.11.2)