FastQCFastQC Report
Thu 21 Jul 2016
H7JJJBGXY_n01_bgm36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJJBGXY_n01_bgm36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15181313
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT14200.015.5307231
CCGTATT16900.014.1093082
GTCCGGT14900.013.1397461
GTCGTTT17150.012.9904751
GCCGTAT13250.012.5681061
GTCGCTT21750.012.4158351
ATTTGCG14250.011.997276
CCTTATG26600.011.9240582
GCCTTAT40050.011.8558821
CGCGACT5800.011.64337530
ACGCGAC5900.011.44482329
TTACGGC7350.011.32533540
TATCCGT13700.011.00201641
GTCCGCA19750.010.8245661
ATCGTAT11450.010.8054026
CGTATTT26900.010.7875083
GTCGGCG25200.010.7160491
TTTGCGG30300.010.6906377
GATCGAA12250.010.6506385
ATTACGG11050.010.588407539
Produced by FastQC (version 0.11.2)