FastQCFastQC Report
Thu 21 Jul 2016
H7JJJBGXY_n01_bgm35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJJBGXY_n01_bgm35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14626989
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT11450.022.2097911
CTCCGGT24900.018.7990321
CCCGGAT17000.018.6655881
GTCGTTT28100.018.6604061
TTACGGC21900.018.59372340
CCCGTTT22350.018.4265921
ATTACGG22500.018.2998739
GTCCGGT12300.018.2964321
CCCCGCT22250.017.4979651
TCCGGTG20150.017.1930872
GCGGAAT15900.016.8430731
GTCCGCT16150.016.5823441
CCCGTCT25150.016.285641
GCTCCGT23950.016.0680081
GGCCGCG14950.015.8059131
TGTCGGA25450.015.64784224
CCCCGAT13850.015.5988641
CCCGTGT20850.015.5427521
CCCATAT34750.015.4779911
GCCCGTT16000.015.4719191
Produced by FastQC (version 0.11.2)