FastQCFastQC Report
Thu 21 Jul 2016
H7JJJBGXY_n01_bgm34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJJBGXY_n01_bgm34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14273622
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT12350.018.2213941
CCGTATT16000.015.6064432
CTCCGGT31750.014.2462621
CCCGTTT24250.013.91964151
CCCGTCT29600.013.8365641
CCCGATT8700.013.7089811
TATCCGT12950.013.20275541
GTATCCG13650.012.6906840
CGTATTT27550.012.6563823
ATTTGCG16000.012.6537856
TCCGGTG28550.012.607092
CCCGGAT37500.012.2418611
CGCGACT5950.012.10611630
ACGCGAC5600.012.05696829
CTCGTAT23300.011.8794891
CCCGTAC8850.011.6966941
GACGCGA5800.011.64076228
CCCGAAT27150.011.6039741
CCGGTGT29500.011.2859763
CCCCGAT17700.011.1881421
Produced by FastQC (version 0.11.2)