FastQCFastQC Report
Thu 21 Jul 2016
H7JJJBGXY_n01_bgm33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJJBGXY_n01_bgm33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459822134
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT403700.016.907521
TTACGGC292300.014.71620740
CCTTATG899550.014.4970112
GTCGTTT683700.013.7126041
CCGTATT432250.013.63536552
ATTACGG368950.013.27011839
GTACGCT265050.013.0415631
GTCGCTT682250.012.9797821
GCCTTAT1391100.012.7186481
GTCCGGT427000.012.5170491
CCCGTCT924850.012.380611
GTCGGTT630800.012.2225521
GTCCGTT533150.011.861051
TATCCGT371150.011.80152341
CTCCGGT962100.011.6369491
ACGCGAC172550.011.50425629
GTACGTA209300.011.50136517
CGCGACT178150.011.49800430
GTTCGCT525150.011.3775151
GTCCGCT791100.011.3673791
Produced by FastQC (version 0.11.2)