FastQCFastQC Report
Thu 21 Jul 2016
H7JJJBGXY_n01_bgm32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJJBGXY_n01_bgm32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16390276
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGA27600.027.40366430
ATCGGAA31100.024.1591822
ACACGTC32050.023.44271913
CGTCTGA32800.022.7021116
TATCGCG4950.022.2738739
ACGTCTG35800.021.3018915
TCGTATG23750.021.02752543
GATCGGA35650.020.010481
GTCACCG38500.019.64204829
CACACGT38600.019.46474512
CACCGAT41600.018.23594531
TATCTCG26900.018.23513639
CCCGTAT18450.018.0519081
CGATGTA42250.017.95544634
GATGTAT42050.017.82660335
CGTATGC28850.017.62230744
CACGTCT42950.017.59809514
ATGTATC44550.016.82597736
GTATGCC31550.016.54192745
CCGTATT17200.016.2177052
Produced by FastQC (version 0.11.2)