FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68625826
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20912403.047307583591052No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGG997070.1452907830938166No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG832240.1212721286589687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTC82100.084.282269
GGACCTC53600.080.009729
GGCAATG68250.077.865889
GGCTAAG76050.073.18949
GTATAGC45250.071.369089
GGGAACA62150.070.836719
GTAACTC57550.070.226989
GGGACCT30250.070.0095148
TTAGGCT70300.069.920549
TGAACTC70300.069.717879
GGGAACT41050.068.363128
TGACCTC54150.068.234029
CTCGCTG65100.067.772669
GGCTGAG117800.067.4885259
GGACGTA53950.067.166529
GTGTCGT60250.067.079889
AGCGCTG56950.065.5463649
GTCATAC58800.065.341799
TAATTGG62350.065.201429
GTACTAT46050.064.766419
Produced by FastQC (version 0.11.5)